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Briefly, maturbation l of bined cell lysis and assay reagent buffer were added directly to the well using a matrix wellmate (thermo scientific) after agitating plates for min.

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Addition of l of dilution buffer ( m m tris-hcl, m m nacl, lacy chabert ph, nicole lunders m m dithiothreitol, m m edta, % nonidet p-40, % glycerol, % me so) to each well by using a wellmate.

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